Abstract:
Studies have linked herbal teas to the prevention of many diseases, leading to an increase in 
their use and demand which has endorsed an increase in their commercial activity and 
production. Consequently, performing a quality assessment of herbal teas in Botswana to 
determine their safety, medicinal and economical value has become of greater importance. The 
quality assessment tools used in this study entail: Antioxidant activity profiling using 2,2-
diphenyl-1-picrylhydrazyl (DPPH), Total phenolic content (TPC) and metal chelation activity 
as well as proximate and metal content analysis. The brewing conditions of 80o C at 60 minutes 
gave the best antioxidant activity at the lowest DPPH IC50 value of 0.1600 ± 0.0489 µg/mL 
and the highest TPC of 12319.880 ±0.622 mg GAE/g. The proximate analysis showed that 
Terminalia prunioides pods contained ash 3.8816 ± 0.1254%, moisture 9.3265 ± 1.5362 %, 
Crude fiber 7.5908 ± 1.4409 %, and protein 0.00662 ± 0.000717%. The metal content profile 
showed the presence of the following essential metal elements potassium 10078 ± 325.0 mg/L, 
calcium 8930 ± 875.1 mg/L, sodium 722.4 ± 250.2 mg/L, and magnesium 2205 ± 65.10 mg/L. 
The heavy metals present where; Arsenic 1.310 ± 0.08020, chromium 2.380 ± 0.7650 mg/L, 
copper 16.01 ± 0.3503 mg/L, manganese 28.70 ± 0.6001 mg/L, nickel 0.7600 ± 0.1210 mg/L, 
lead 4.245 ± 1.575 mg/L, Fe 306.5 ± 1.610, cobalt 1.290 ± 0.1550 mg/L and zinc 1.413 ± 
0.6002 mg/L. The elements that were found at permissible values are K, Ca, Mg, Na, Mn, Ni,
and Fe, whilst As, Cu, Cr, Co, Zn, and Pb were found at higher values. The tea contains 
desirable essential minerals such as K, Mg, Ca, Na, Mn, Fe, and Ni and is an excellent source 
of polyphenolic components that can be explored as a source of dietary antioxidants. The 
proximate analysis data is also sufficient for herbal tea to be considered as a food product. All 
three profiles provided preliminary scientific data to ensure the quality and efficacy of 
Terminalia prunioides pods as herbal tea. Therefore, this phytochemistry study shows that 
Terminalia prunioides pods have the potential to be used in herbal tea formulations.