Water disinfection is a crucial water treatment process because it deals with the removal of pathogens to make water safe to drink. Moringa oleifera extract was used as a disinfectant and the total coliform count was monitored through microscopic observations. The total coliform bacteria removal at a dosage of 5 ml/100 ml of Moringa Oleifera extract was counted to be 13 colonies from an original count of 200 colonies, translating to about 93.5% removal of the bacterial strains. The results showed that MO extract dosages higher than 5 ml/100 ml remove lesser bacteria due to an increase in organic matter. Moringa oleifera seeds have shown the ability to act as a disinfectant by reducing the total coliform bacteria in waste water by 93.5% at an extract dosage of 5 ml/100 ml proving that it has anti-microbial properties.