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Prediction of genes that function in methanogenesis and CO2 pathways in extremophiles

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dc.contributor.author Tilahun, Lulit
dc.contributor.author Asrat, Asfawossen
dc.contributor.author Wessel, Gary M.
dc.contributor.author Simachew, Addis
dc.date.accessioned 2022-06-28T10:16:09Z
dc.date.available 2022-06-28T10:16:09Z
dc.date.issued 2021-10-24
dc.identifier.citation Tilahun, L. et al. (2021) Prediction of genes that function in methanogenesis and CO2 pathways in extremophiles. Geosciences, 9 (11), 2211. https://doi.org/10.3390/microorganisms9112211. en_US
dc.identifier.issn 2076-2607
dc.identifier.uri http://repository.biust.ac.bw/handle/123456789/462
dc.description.abstract Gaet’ale (GAL) and Mud’ara (MUP) are two hypersaline ponds located in the Danakil Depression recharged by underground water from the surrounding highlands. These two ponds have different pH, salinity, and show variation in the concentration of many ionic components. Metagenomic analysis concludes that GAL is dominated by bacteria as in the case of the other hypersaline and acidic ponds in the Danakil Depression. However, Archaea dominated the ponds of MUP. In the current study, the application of SEED and KEGG helped to map the ordered steps of specific enzyme catalyzed reaction in converting CO2 into cell products. We predict that highly efficient and light-independent carbon fixation involving phosphoenolpyruvate carboxylase takes place in MUP. On the contrary, genes encoding enzymes involved in hydrogenotrophic and acetoclastic methanogenesis appeared solely in ponds of GAL, implying the biological source of the hazardous methane gas in that environment. Based on the investigation of the sources of the genes of interest, it is clear that cooperative interactions between members of the two communities and syntrophic metabolism is the main strategy adapted to utilize inorganic carbon as a carbon source in both MUP and GAL. This insight can be used to design biotechnological applications of microbial communities in production of methane biogas or to minimize CO2 emissions. en_US
dc.description.sponsorship Support by National Institutes of Health USA through grant NIH 1R35GM140897. en_US
dc.language.iso en en_US
dc.publisher MDPI en_US
dc.subject Extremophiles en_US
dc.subject Gaet’ale en_US
dc.subject Mud’ara en_US
dc.subject Danakil Depression en_US
dc.subject Ethiopia syntrophic metabolism en_US
dc.subject Methanogenesis en_US
dc.subject Biotechnology en_US
dc.title Prediction of genes that function in methanogenesis and CO2 pathways in extremophiles en_US
dc.description.level phd en_US
dc.description.accessibility unrestricted en_US
dc.description.department mge en_US


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